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Frequently
Asked Questions: TBS-380 Fluorometer
Note: If
your question is not answered in this document, we encourage you to contact
us toll free at 888-636-2401 or 408-636-2400 x 119. You may also use our
online contact form. Your question
will be answered by one of our trained sales, technical, or applications
specialists.
What is
the price of the TBS-380 Fluorometer?
The price may vary based on your application(s) and requirements. To receive
a quote customized to your needs, use our online quotation form (click
here).
What are
the primary applications of the TBS-380 Fluorometer?
The primary application for the TBS-380 Fluorometer are nucleic acid and
protein quantitation. A list of the applications include:
DNA quantitation:
PicoGreen® and Hoechst 33258
RNA quantitation: RiboGreen®
Protein quantitation: NanoOrange™
What is
the sensitivity of the TBS-380 Fluorometer?
The TBS-380's sensitivity is defined in terms of the minimum detection
limits of dsDNA and RNA. The TBS-380 Fluorometer can detect:
|
|
Minimum
Detection Limit |
|
Analyte |
Using
a 10x10 mm Cuvette |
Using
a Minicell |
|
| DNA
- PicoGreen® |
500
pg/mL |
1
ng/mL |
| DNA
- Hoechst 33258 Dye |
10
ng/mL |
10
ng/mL |
| RNA
- RiboGreen® |
1
ng/mL |
1
ng/mL |
| Protein
- NanoOrange™ |
100
ng/mL |
N/A |
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What is
the optical configuration of the TBS-380 Mini-Fluorometer?
The TBS-380 has two dedicated optical channels, UV and BLUE channels.
UV: Ex 365-395 nm, Em 440-470 nm
BLUE: Ex 465-485 nm, Em 515-575 nm
What size
sample tube/cuvette is standard with the TBS-380 Fluorometer?
The standard cuvette size for the TBS-380 Fluorometer is 10x10 mm plastic
cuvettes. With the optional adaptors, like the Minicell Adaptor, other
cuvettes can be used.
What is
the Minicell Adaptor?
The TBS-380 Minicell Adaptor is designed to accommodate the measurement
of small volume samples. The design of the focusing lenses allows you
to measure fluorescence samples in a much reduced assay volume without
losing sensitivity.
Do the
sample tubes/cuvettes require minimum volumes? If so, what are they?
Sample tubes do require minimum volumes so that enough sample is exposed
to the incoming light. The minimum volumes are as follows:
|
Cuvette/Tube |
Adaptor
Needed? |
Minimum
Volume |
|
10
x 10 mm |
No |
2.0
mL |
|
Minicell |
Yes |
50
µl |
What kind
of cuvettes should I use with the TBS-380 Fluorometer?
Methacrylate or polystyrene 10x10 mm cuvette, or 10x10 mm adaptor that
is designed for fluorescence measurement is appropriate. Due to the natural
fluorescence properties of polystyrene, this material is not recommended
for UV applications. The following sample tubes and cuvettes are available
for purchase from Turner BioSystems:
P/N 7000-957
10 x 10 mm Square Polystyrene Cuvette (3.5 mL)
P/N 7000-959 10 x 10 mm Square Methacrylate Cuvette (3.5 mL)
Minicell Borosilicate Glass (75-250 mL)
Can I
use a quartz or glass cuvette?
The sample compartment cannot accept glass or quartz cuvettes.
Does the
TBS-380 Fluorometer have changeable optical filters?
No, the TBS-380 Fluorometer does not have changeable optical filters.
If the TBS-380 Fluorometer's optical configuration does not fit your application
requirement, please see information about the
TD-700 Fluorometer.
What is
the dynamic range of the TBS-380 Fluorometer?
The dynamic range of the TBS-380 Fluorometer is about three orders of
magnitude for most of the applications.
Was your
question answered?
If not, contact us.
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