Frequently Asked Questions: Veritas Microplate Luminometer

Note: If your question is not answered in this document, we encourage you to contact us toll free at 888-636-2401 or 408-636-2400 x 119. You may also use our online contact form. Your question will be answered by one of our trained sales, technical, or applications specialists.

What is the price of the Veritas Microplate Luminometer?
The price may vary based on your application(s) and requirements. To receive a quote customized to your needs, use our online quotation form (click here).

What is the detection system of the Veritas™ Microplate Luminometer?
The Veritas™ has a photomultiplier tube (PMT) detector. Because of its proprietary design, this detector offers both superior sensitivity and dynamic range.

How many injectors can be installed in the Veritas™ Microplate Luminometer?
There are total of two optional injectors that can be installed on the Veritas™ Microplate Luminometer.

What are the advantages of internal automatic injectors versus external automatic injectors?
The Veritas™ internal automatic injectors permit dark-adaptation of the injectable reagents. The reagent supply remains in a protected, dark space inside the Veritas™ during the experiment. Because many reagents are light sensitive, equilibrating the reagents to dark conditions allows for maximum reproducibility. Other microplate luminometers rely on external automatic injectors, which draw reagents from a supply outside of the luminometer and do not protect reagents from the influence of ambient light. Internal injectors also shorten the supply line length and reduce dead volume.

What kind of microplates can the Veritas™ Microplate Luminometer read?
The Veritas™ can read any opaque white or black 96-well plates. It can also read opaque wall clear bottom plates when used with a bottom plate sealer (P/N 6570 Costar).

Is there a recommended brand of 96-well plates that I should use?
Our tests show the best sensitivity is achieved using Greiner solid white assay plates, 96-well medium binding, Lumitrac 200. These plates can be purchased through E&K Scientific (Cat # EK25075) in cases of 40 plates.

What operating software does Veritas™ come with?
The Veritas™ has a PC based Direct-to-Excel software. The software operates the instrument and reports the data directly to an Excel spreadsheet.

What operating systems are compatible with the Veritas™ software?
The Veritas™ software is compatible with Microsoft Windows® 95 or later.

Can I do kinetic measurements?
Yes, the current version of the software supports kinetic measurement.

Is the software compatible with Macintosh® operating systems?
No, it is not compatible with Macintosh operating systems.

What applications can I use this instrument for?
You can run all bio- and chemiluminescent, flash and glow type of applications on the Veritas™. The primary applications are: Reporter gene assays, ATP assays, Immunoassays, Cytotoxicity, Cell proliferation, Nucleic acid quantitation, Enzyme assays, Intracellular Ca2+

Can I use the Veritas™ for flash reactions?
Yes, you can run flash reactions if the Veritas™ has injector(s) installed.

What is the sensitivity of the Veritas™ Microplate Luminometer?
The Veritas™ has a detection limit of 3x10-21 moles luciferase using Promega's luciferase assay reagent.

How does the sensitivity of the Veritas™ compare to other luminometers?
The sensitivity of the Veritas™ is 10 times higher than the leading competitor's instrument. The Veritas™ is also comparable in sensitivity to the TD-20/20 Single Tube Luminometer.

What is the dynamic range of the Veritas™?
The Veritas™ has over 9 decades of dynamic range.

How does the dynamic range of the Veritas™ compare to other instruments?
Other instruments on the market usually have dynamic range of 6 decades.

What is the injection volume and precision of the Veritas™?
The injection volume is selectable between 25 and 250 µL (±1 µL); CV% <1%

What is the crosstalk of the Veritas™?
The crosstalk of the Veritas™ is less than 3x10-5 with a white 96-well plate. When using a black plate the crosstalk is less than 3x10-7.

Do I need to change anything for power requirements in different countries?
No, the Veritas™ has an internal universal power supply. Simply use the proper AC adapter.

What is the Veritas™ Microplate Luminometer Light Plate?
The light plate is designed to provide a quick and easy way to verify the performance of the Veritas™. It allows the user to check the reproducibility, sensitivity, and linearity of a luminometer in less than 1 minute. The light plate consists of three highly stable light sources that simulate luminescence samples at signal levels spreading over four decades. It can be used for both short and long term stability studies of the detection system.

What software do I need on my PC?
The Veritas™ software is provided on a CD and is easy to install. You only need to have an available serial port to connect to the Veritas™. You must have MS Excel installed on your computer.

Does the instrument attach to a printer?
No, the printer does not directly connect to the Veritas™. Instead, the printer attaches to the PC that is connected to the Veritas™.

Does the Veritas™ software work with older versions of Windows® software?
We were able to test the Veritas™ software on Windows® 95. The Veritas™ software was successful in translating to MS Excel.

Was your question answered?
If not, contact us.