"I have been using the Turner BioSystems fluorometer to measure DNA amounts from my chromatin immunoprecipitation methods. This instrument is perfect for our applications. It has a very small footprint and works like a charm when hooked up to the computer. Life in the lab would be very different without the instrument."
Tae Hoon Kim, Yale University School of Medicine

Modulus™ Single Tube Fluorometer

The Concept

The Modulus™ Single Tube Fluorometer is designed to provide utmost flexibility. The Modulus™ Single Tube operates as a dedicated fluorometer, or a combination fluorometer/luminometer. With the optional Absorbance Module, absorbance measurements are available in the visible wavelength spectrum. Modulus allows you to buy what you need now, and to add what you want later.

Fluorescence

Fluorescent measurements with the Modulus are simple. Several optical kits are available for the most common fluorescent applications. The optics are factory installed into an Optical Kit that snaps into the Modulus. Each kit contains a light source, an excitation filter, and an emission filter to yield the highest performance.

Custom Optical Kits are available upon request.

Nucleic Acid Quantitation

The Modulus provides high sensitivity for fluorescent measurements of DNA and RNA. There are two options for measuring nucleic acids on the Modulus. Quantitate DNA via PicoGreen or Hoechst or quantitate RNA via RiboGreen or Quant-iT RNA. You can detect as little as 4.5 pg of dsDNA in 100 ul using PicoGreen and the Minicell Adaptor. Use the Minicell Adaptor to minimize reagent volume and cost.

Protein Quantitation

The Modulus provides flexibility for protein measurement. The two methods for measuring proteins on the Modulus are fluorescence or absorbance. Quantiate protein via Quant-iT Protein Assay using a Fluorescence Optical Kit. Alternatively, the Bradford, Lowry, and BCA assays are possible with the Absorbance Module.

Gene Expression

The Modulus provides flexibility for gene expression measurements. The two methods for measuring gene expression on the Modulus are fluorescence or luminescence. As a fluorometer, the Modulus is capable of measuring several different GFP variants.

Luminescence

The Luminescence Module accommodates luminescent assays such as ATP measurements, gene expression using luciferase, or other bio- and chemiluminescent measurements. Change the module to switch between fluorescence and luminescence.

Gene Expression

The Modulus provides flexibility for gene expression measurements. The two methods for measuring gene expression on the Modulus are fluorescence or luminescence. As a luminometer, the Modulus analyzes luciferase expression coupled to gene expression.

ATP Measurements and Reactive Oxygen Species (ROS)

The Modulus as a luminometer measures cell viability through the use of ATP determination. Cell viability can also be measured using the ATP-dependent oxidation of luciferase. ROS assays that utilize chemiluminescent substrates such as luminol can also be measured on the Modulus.

Absorbance

A snap-in Absorbance Module facilitates absorbance measurements. For example, measure protein concentration using standard assays such as Bradford, BCA, or Lowry. Filter paddles let you select the right wavelength for your assay.

Protein Quantitation

The Modulus provides flexibility for protein measurement. The two methods for measuring proteins on the Modulus are fluorescence or absorbance. The Bradford, Lowry, and BCA assays are possible with the Absorbance Module.

Modulus Single Tube General Specifications

General Specifications

Data Output: 100% ASCII format through a 9-pin RS-232 serial cable at 9600 baud
User Interface: Requires Windows® 98 or later
Power: 12V 0.84A Max
Dimensions: 12.92'' D x 10.44'' W x 8.42" H (32.82 cm D x 26.52 cm W x 21.39 cm H)
Weight: 8.1 lbs (3.65 kg)
Operating Temperature: 60-105 °F (15-40 °C)
Warranty: One year
Approvals: CE

Modulus Fluorometer Specifications

Fluorescence Module Performance

Sensitivity: 6 ppt fluorescein, 4.5 pg DNA with PicoGreen Dye
Dynamic Range: 6
Detector: Photodiode
Light Source: UV LED 365-395 nm, Blue LED 465-485 nm, Green LED
495-525 nm, Red LED 620-645 nm
Sample Adaptor: Holds 10 x 10 mm cuvette or minicell vial (using the Minicell Kit)
Read Out: Direct concentration or Raw fluorescence
Calibration: 1 to 5 point calibration
Discrete Sample Average: Sample readings are averaged to improve accuracy

GSA Contract # GS 24F 0056N

For research use only. Not for use in diagnostic procedures.
Modulus is a trademark of Turner BioSystems, Inc. All other trademarks are the sole property of their respective owners.